{"id":7772,"date":"2026-04-17T22:54:04","date_gmt":"2026-04-17T13:54:04","guid":{"rendered":"https:\/\/tuat-chemphys.net\/?p=7772"},"modified":"2026-04-22T07:23:50","modified_gmt":"2026-04-21T22:23:50","slug":"%e9%9b%bb%e5%ad%90%e7%b7%9a%e3%81%ab%e3%81%a9%e3%81%93%e3%81%be%e3%81%a7%e8%80%90%e3%81%88%e3%82%89%e3%82%8c%e3%82%8b%ef%bc%9f%e8%9b%8d%e5%85%89%e3%82%bf%e3%83%b3%e3%83%91%e3%82%af%e8%b3%aa%e3%81%ae","status":"publish","type":"post","link":"https:\/\/tuat-chemphys.net\/?p=7772","title":{"rendered":"\u96fb\u5b50\u7dda\u306b\u3069\u3053\u307e\u3067\u8010\u3048\u3089\u308c\u308b\uff1f\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u65b0\u3057\u3044\u53ef\u80fd\u6027 Investigation of electron beam resistance of a fluorescent protein using an electron photon hybrid microscope"},"content":{"rendered":"\n<p class=\"has-black-color has-pale-ocean-gradient-background has-text-color has-background has-link-color wp-elements-a7ff8a791edc5e8f3483e521810601a3 wp-block-paragraph\">\u79c1\u305f\u3061\u306f\u3001\u751f\u304d\u305f\u7d30\u80de\u306e\u4e2d\u3092\u300c\u5149\u300d\u3067\u898b\u308b\u305f\u3081\u306b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f7f\u3063\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3001\u96fb\u5b50\u9855\u5fae\u93e1\u306f\u3068\u3066\u3082\u7d30\u304b\u3044\u69cb\u9020\u307e\u3067\u898b\u3048\u307e\u3059\u304c\u3001\u5f37\u3044\u96fb\u5b50\u7dda\u306b\u3088\u3063\u3066\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u58ca\u308c\u3066\u3057\u307e\u3046\u3068\u3044\u3046\u554f\u984c\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u7814\u7a76\u3067\u306f\u3001<strong>mAzamiGreen\uff08mAG\uff09<\/strong>\u3068\u3044\u3046\u69cb\u9020\u304c\u5f37\u3044\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u6ce8\u76ee\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u96fb\u5b50\u7dda\u3092\u5f53\u3066\u308b\u3068\u5149\u306f\u5f31\u304f\u306a\u308a\u307e\u3059\u304c\u3001mAG\u306f\u3088\u304f\u4f7f\u308f\u308c\u308bEGFP(\u9752\u8272\u3084\u7d2b\u5916\u7dda\u306e\u4e0b\u3067\u7dd1\u8272\u306b\u5149\u308b\u30bf\u30f3\u30d1\u30af\u8cea)\u3088\u308a10\u500d\u4ee5\u4e0a\u3082\u9577\u304f\u8010\u3048\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u6c34\u306e\u4e2d\u3067\u306f\u5206\u5b50\u304c\u5c11\u3057\u4fee\u5fa9\u3055\u308c\u308b\u305f\u3081\u3001\u4e7e\u71e5\u72b6\u614b\u3088\u308a\u3082\u58ca\u308c\u306b\u304f\u3044\u3053\u3068\u3082\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u6210\u679c\u306f\u3001\u5149\u3068\u96fb\u5b50\u3092\u540c\u6642\u306b\u4f7f\u3063\u3066\u7d30\u80de\u3092\u898b\u308b\u65b0\u3057\u3044\u9855\u5fae\u93e1\u6280\u8853\u306b\u3064\u306a\u304c\u308b\u3001\u5927\u304d\u306a\u4e00\u6b69\u3067\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\">&#x1f9ec; mAzamiGreen \u306f\u3069\u3053\u307e\u3067\u8010\u3048\u3089\u308c\u308b\uff1f<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">GFP (Green fluorescent protein) \u306f\u3001\u9752\u8272\u3084\u7d2b\u5916\u7dda\u306e\u4e0b\u3067\u7dd1\u8272\u306b\u5149\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u3002\u672c\u7814\u7a76\u3067\u306f\u3001\u5f93\u6765\u3088\u304f\u7814\u7a76\u3055\u308c\u3066\u304d\u305f <strong>EGFP<\/strong>\uff08enhanced GFP: \u91ce\u751f\u578b GFP \u306e\u5909\u7570\u4f53\u3067\u3001GFP \u306b\u5bfe\u3057\u3066\u3088\u308a\u9ad8\u8f1d\u5ea6\u306e\u767a\u5149\u3092\u793a\u3059\uff09 \u3088\u308a\u3082<strong>\u69cb\u9020\u7684\u306b\u5b89\u5b9a<\/strong>\u3068\u3055\u308c\u308b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea<strong>mAzamiGreen\uff08mAG\uff09<\/strong> \u306b\u6ce8\u76ee\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u305d\u306e\u7d50\u679c\u3001\u96fb\u5b50\u7dda\u7167\u5c04\u306b\u3088\u3063\u3066\u3001(1) \u86cd\u5149\u5f37\u5ea6\u306f\u6e1b\u5c11\u3059\u308b\u3001(2) \u767a\u5149\u30b9\u30da\u30af\u30c8\u30eb\u306f\u8d64\u5074\u306b\u30b7\u30d5\u30c8\u3059\u308b\u3002\u3057\u304b\u3057\u3001<strong>mAG \u306f EGFP \u3088\u308a1\u6841\u4ee5\u4e0a\u9ad8\u3044\u96fb\u5b50\u7dda\u8010\u6027<\/strong>\u3092\u793a\u3059\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">&#x1f4a1; \u306a\u305c\u8010\u6027\u304c\u9ad8\u3044\u306e\u304b\uff1f<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">mAG \u306b\u306f\u3001<strong>\u767a\u5149\u4e2d\u5fc3\uff08\u30af\u30ed\u30e2\u30d5\u30a9\u30a2\uff09\u3068\u30d2\u30b9\u30c1\u30b8\u30f3\u6b8b\u57fa\u306e \u03c0\u30b9\u30bf\u30c3\u30ad\u30f3\u30b0\u69cb\u9020<\/strong>\u3068\u3044\u3046\u3001EGFP\u306b\u306f\u306a\u3044\u7279\u5fb4\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u69cb\u9020\u304c\u3001\u96fb\u5b50\u7dda\u306b\u3088\u308b\u5206\u89e3\u3092\u53d7\u3051\u306b\u304f\u3044\u3088\u308a\u201c\u58ca\u308c\u306b\u304f\u3044\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u201d\u3092\u5b9f\u73fe\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">&#x1f331; \u4e7e\u71e5\u72b6\u614b\u3068\u6eb6\u6db2\u4e2d\u306e\u9055\u3044<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u3055\u3089\u306b\u672c\u7814\u7a76\u3067\u306f\u3001<strong>\u4e7e\u71e5\u72b6\u614b<\/strong>\u3088\u308a\u3082\u6eb6\u6db2\u4e2d\uff08\u6c34\u4e2d\uff09\u306e\u307b\u3046\u304cmAG \u306e\u96fb\u5b50\u7dda\u8010\u6027\u304c\u3084\u3084\u9ad8\u3044\u3053\u3068\u3082\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001\u6eb6\u6db2\u4e2d\u3067\u306f\u5206\u5b50\u304c\u5468\u56f2\u3068\u4ea4\u63db\u3055\u308c\u3001<strong>\u69cb\u9020\u306e\u201c\u81ea\u5df1\u4fee\u5fa9\u201d\u304c\u90e8\u5206\u7684\u306b\u8d77\u304d\u3066\u3044\u308b\u53ef\u80fd\u6027<\/strong>\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading\">&#x1f9ec; Why mAzamiGreen?<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">In this study, Minoda Lab investigated <strong>mAzamiGreen (mAG)<\/strong>, a fluorescent protein known for its <strong>high structural stability<\/strong>, and compared it with the widely used EGFP. Electron\u2011beam irradiation caused: (1) a decrease in luminescence intensity, and (2) a red shift of the emission peak in both proteins. However, a key finding is that <strong>mAG survives electron irradiation more than one order of magnitude better than EGFP<\/strong>.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">&#x1f4a1; Origin of the enhanced resistance<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">mAG possesses a unique <strong>\u03c0\u2011stacking interaction<\/strong> between its chromophore and a histidine residue, a feature absent in EGFP. This structural robustness is considered responsible for the <strong>slower intensity decay and improved electron\u2011beam resistance<\/strong>.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">&#x1f4a7; Dry vs. wet environments<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">The experiments further revealed that mAG is slightly more resistant: in solution than in the dry state. This suggests that molecular exchange with the surrounding environment may allow partial structural repair under irradiation.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">&#x1f680; Future potential<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">These results indicate that mAzamiGreen is a promising candidate for: (i) cathodoluminescence\u2011based correlative microscopy, (ii) multi\u2011color CL imaging, and (iii) real\u2011time observation of biological structures at nanometer resolution.<\/p>\n\n\n\n<p class=\"has-white-color has-accent-background-color has-text-color has-background has-link-color wp-elements-3491e9e1504bd6877168b6e59e8d73ba wp-block-paragraph\">JOURNAL PAPER: Micron<br>Volume 201, February 2026, 103944<br>Investigation of electron beam resistance of mAzamiGreen using an electron photon hybrid microscope<br>Mihiro Suzuki, Toshiki Shimizu, Hiroki Minoda<br><a href=\"https:\/\/doi.org\/10.1016\/j.micron.2025.103944\">https:\/\/doi.org\/10.1016\/j.micron.2025.103944<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Fluorescent proteins allow scientists to \u201csee\u201d what is happening inside living cells using light. Electron microscopes, on the other hand, can observe extremely fine structures, but strong electron beams often damage fluorescent proteins. In this study, Minoda Lab focused on mAzamiGreen (mAG), a structurally stable fluorescent protein. They found that although electron beams reduce its brightness, mAG survives more than ten times longer than the commonly used EGFP. They also discovered that mAG is slightly more resistant in liquid than in dry conditions, likely because molecules can partially repair their structure. These results open the door to next\u2011generation microscopes that combine light and electron imaging in real time.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"has-white-color has-highlight-background-color has-text-color has-background has-link-color wp-elements-8909d3738d159f1f79ce0846237c1453 wp-block-paragraph\">\u672c\u8a18\u4e8b\u306f\u3001\u516c\u958b\u6e08\u307f\u8ad6\u6587\u306b\u57fa\u3065\u304d\u5b66\u79d1\u5e83\u5831\u7528\u306b\u7de8\u96c6\u3057\u305f\u7d39\u4ecb\u6587\u3067\u3059\u3002This article is an outreach-style summary prepared for the department website based on the published paper.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>mAzamiGreen\u306e\u65b0\u3057\u3044\u53ef\u80fd\u6027\u3092\u89e3\u660e\u3002mAzamiGreen shows remarkable robustness.<\/p>\n","protected":false},"author":1,"featured_media":7773,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"jetpack_seo_schema_type":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[9],"tags":[16],"class_list":["post-7772","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research","tag-measurement-devices"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/tuat-chemphys.net\/wp-content\/uploads\/2026\/04\/Designer-3.png","jetpack_sharing_enabled":true,"jetpack-related-posts":[{"id":6478,"url":"https:\/\/tuat-chemphys.net\/?p=6478","url_meta":{"origin":7772,"position":0},"title":"Eco-friendly AgBiS\u2082 nanocrystals with photodetector performance. \u74b0\u5883\u306b\u3084\u3055\u3057\u3044AgBiS\u2082\u30ca\u30ce\u7d50\u6676\u3092\u958b\u767a\u3001\u5149\u96fb\u5b50\u30c7\u30d0\u30a4\u30b9\u306e\u672a\u6765\u306b\u671f\u5f85","author":"unet","date":"2025-08","format":false,"excerpt":"\u4eca\u5f8c\u306f\u3001\u30ca\u30ce\u7d50\u6676\u306e\u30b5\u30a4\u30ba\u5236\u5fa1\u6280\u8853\u306e\u958b\u767a\u3092\u9032\u3081\u3001\u74b0\u5883\u306b\u914d\u616e\u3057\u305f\u9ad8\u6027\u80fd\u5149\u96fb\u5b50\u30c7\u30d0\u30a4\u30b9\u306e\u5b9f\u73fe\u3092\u76ee\u6307\u3057\u307e\u3059\u3002Future work will focus on developing size control techniques for nanocrystals to realize environmentally friendly, high-performance optoelectronic devices.","rel":"","context":"In &quot;Research Topics&quot;","block_context":{"text":"Research Topics","link":"https:\/\/tuat-chemphys.net\/?cat=9"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2025\/08\/image.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2025\/08\/image.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2025\/08\/image.png?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":7779,"url":"https:\/\/tuat-chemphys.net\/?p=7779","url_meta":{"origin":7772,"position":1},"title":"\u5f53\u5b66\u79d1\u306e\u300c\u91cf\u5b50\u6280\u8853\u300dQuantum technology research at our department","author":"unet","date":"2026-04","format":false,"excerpt":"\u91cf\u5b50\u306e\u4e16\u754c\u306f\u3001\u7269\u7406\u3084\u5316\u5b66\u306e\u300c\u5f53\u305f\u308a\u524d\u300d\u304c\u901a\u3058\u306a\u3044\u4e0d\u601d\u8b70\u306a\u5834\u6240 The \"Quantum-Tech\" tag, highlighting our lineup of researchers pushing the boundaries of what is scientifically possible.","rel":"","context":"In &quot;Lab. Intro \u7814\u7a76\u5ba4\u7d39\u4ecb&quot;","block_context":{"text":"Lab. Intro \u7814\u7a76\u5ba4\u7d39\u4ecb","link":"https:\/\/tuat-chemphys.net\/?cat=6"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/04\/Gemini_Generated_Image_tnsv0ctnsv0ctnsv.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/04\/Gemini_Generated_Image_tnsv0ctnsv0ctnsv.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/04\/Gemini_Generated_Image_tnsv0ctnsv0ctnsv.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/04\/Gemini_Generated_Image_tnsv0ctnsv0ctnsv.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/04\/Gemini_Generated_Image_tnsv0ctnsv0ctnsv.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/04\/Gemini_Generated_Image_tnsv0ctnsv0ctnsv.png?resize=1400%2C800&ssl=1 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&quot;Awards&quot;","block_context":{"text":"Awards","link":"https:\/\/tuat-chemphys.net\/?cat=3"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/06\/image.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/06\/image.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/06\/image.png?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":6688,"url":"https:\/\/tuat-chemphys.net\/?p=6688","url_meta":{"origin":7772,"position":3},"title":"\u539f\u5b50\u30b9\u30d4\u30f3\u306e\u6e2c\u5b9a\uff1a\u30bb\u30b7\u30a6\u30e0\u539f\u5b50\u3078\u306e\u65b0\u305f\u306a\u6d1e\u5bdf Measuring the Atomic Spins: New Insights into Cesium Atoms","author":"unet","date":"2025-09","format":false,"excerpt":"\u5438\u53ce\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u624b\u6cd5\u3001\u3053\u306e\u6280\u8853\u306f\u91cf\u5b50\u6280\u8853\u306e\u767a\u5c55\u3068\u5b9f\u7528\u5316\u306b\u91cd\u8981\u306a\u8ca2\u732e\u3092\u3059\u308b\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002The absorption monitoring method: This technology is expected to make important contributions to the development and practical application of quantum technologies.","rel":"","context":"In &quot;Research Topics&quot;","block_context":{"text":"Research Topics","link":"https:\/\/tuat-chemphys.net\/?cat=9"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2025\/09\/image-1.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2025\/09\/image-1.png?resize=350%2C200&ssl=1 1x, 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Abe&#8221;","author":"unet","date":"2026-03","format":false,"excerpt":"\u8a18\u4e8b\u3067\u306f\u7814\u7a76\u306e\u918d\u9190\u5473\u3084\u3001\u80b2\u5150\u3068\u7814\u7a76\u3092\u4e21\u7acb\u3057\u306a\u304c\u3089\u6b69\u3093\u3067\u304d\u305f\u30ad\u30e3\u30ea\u30a2\u306b\u3064\u3044\u3066\u3082\u8a9e\u3063\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f The article also offers an candid look at her journey balancing research with motherhood.","rel":"","context":"In &quot;Professor's Interview&quot;","block_context":{"text":"Professor's Interview","link":"https:\/\/tuat-chemphys.net\/?cat=5"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/03\/image-1.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/03\/image-1.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/03\/image-1.png?resize=525%2C300&ssl=1 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