{"id":7772,"date":"2026-04-17T22:54:04","date_gmt":"2026-04-17T13:54:04","guid":{"rendered":"https:\/\/tuat-chemphys.net\/?p=7772"},"modified":"2026-04-17T23:15:43","modified_gmt":"2026-04-17T14:15:43","slug":"%e9%9b%bb%e5%ad%90%e7%b7%9a%e3%81%ab%e3%81%a9%e3%81%93%e3%81%be%e3%81%a7%e8%80%90%e3%81%88%e3%82%89%e3%82%8c%e3%82%8b%ef%bc%9f%e8%9b%8d%e5%85%89%e3%82%bf%e3%83%b3%e3%83%91%e3%82%af%e8%b3%aa%e3%81%ae","status":"publish","type":"post","link":"https:\/\/tuat-chemphys.net\/?p=7772","title":{"rendered":"\u96fb\u5b50\u7dda\u306b\u3069\u3053\u307e\u3067\u8010\u3048\u3089\u308c\u308b\uff1f\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u65b0\u3057\u3044\u53ef\u80fd\u6027 How Resistant Is a Fluorescent Protein to Electron Beams?"},"content":{"rendered":"\n

\u79c1\u305f\u3061\u306f\u3001\u751f\u304d\u305f\u7d30\u80de\u306e\u4e2d\u3092\u300c\u5149\u300d\u3067\u898b\u308b\u305f\u3081\u306b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f7f\u3063\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3001\u96fb\u5b50\u9855\u5fae\u93e1\u306f\u3068\u3066\u3082\u7d30\u304b\u3044\u69cb\u9020\u307e\u3067\u898b\u3048\u307e\u3059\u304c\u3001\u5f37\u3044\u96fb\u5b50\u7dda\u306b\u3088\u3063\u3066\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u58ca\u308c\u3066\u3057\u307e\u3046\u3068\u3044\u3046\u554f\u984c\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u7814\u7a76\u3067\u306f\u3001mAzamiGreen\uff08mAG\uff09<\/strong>\u3068\u3044\u3046\u69cb\u9020\u304c\u5f37\u3044\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u6ce8\u76ee\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u96fb\u5b50\u7dda\u3092\u5f53\u3066\u308b\u3068\u5149\u306f\u5f31\u304f\u306a\u308a\u307e\u3059\u304c\u3001mAG\u306f\u3088\u304f\u4f7f\u308f\u308c\u308bEGFP(\u9752\u8272\u3084\u7d2b\u5916\u7dda\u306e\u4e0b\u3067\u7dd1\u8272\u306b\u5149\u308b\u30bf\u30f3\u30d1\u30af\u8cea)\u3088\u308a10\u500d\u4ee5\u4e0a\u3082\u9577\u304f\u8010\u3048\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u6c34\u306e\u4e2d\u3067\u306f\u5206\u5b50\u304c\u5c11\u3057\u4fee\u5fa9\u3055\u308c\u308b\u305f\u3081\u3001\u4e7e\u71e5\u72b6\u614b\u3088\u308a\u3082\u58ca\u308c\u306b\u304f\u3044\u3053\u3068\u3082\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u6210\u679c\u306f\u3001\u5149\u3068\u96fb\u5b50\u3092\u540c\u6642\u306b\u4f7f\u3063\u3066\u7d30\u80de\u3092\u898b\u308b\u65b0\u3057\u3044\u9855\u5fae\u93e1\u6280\u8853\u306b\u3064\u306a\u304c\u308b\u3001\u5927\u304d\u306a\u4e00\u6b69\u3067\u3059\u3002<\/p>\n\n\n\n

<\/h3>\n\n\n\n

🧬 mAzamiGreen \u306f\u3069\u3053\u307e\u3067\u8010\u3048\u3089\u308c\u308b\uff1f<\/h3>\n\n\n\n

GFP (Green fluorescent protein) \u306f\u3001\u9752\u8272\u3084\u7d2b\u5916\u7dda\u306e\u4e0b\u3067\u7dd1\u8272\u306b\u5149\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u3002\u672c\u7814\u7a76\u3067\u306f\u3001\u5f93\u6765\u3088\u304f\u7814\u7a76\u3055\u308c\u3066\u304d\u305f EGFP<\/strong>\uff08enhanced GFP: \u91ce\u751f\u578b GFP \u306e\u5909\u7570\u4f53\u3067\u3001GFP \u306b\u5bfe\u3057\u3066\u3088\u308a\u9ad8\u8f1d\u5ea6\u306e\u767a\u5149\u3092\u793a\u3059\uff09 \u3088\u308a\u3082\u69cb\u9020\u7684\u306b\u5b89\u5b9a<\/strong>\u3068\u3055\u308c\u308b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceamAzamiGreen\uff08mAG\uff09<\/strong> \u306b\u6ce8\u76ee\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u305d\u306e\u7d50\u679c\u3001\u96fb\u5b50\u7dda\u7167\u5c04\u306b\u3088\u3063\u3066\u3001(1) \u86cd\u5149\u5f37\u5ea6\u306f\u6e1b\u5c11\u3059\u308b\u3001(2) \u767a\u5149\u30b9\u30da\u30af\u30c8\u30eb\u306f\u8d64\u5074\u306b\u30b7\u30d5\u30c8\u3059\u308b\u3002\u3057\u304b\u3057\u3001mAG \u306f EGFP \u3088\u308a1\u6841\u4ee5\u4e0a\u9ad8\u3044\u96fb\u5b50\u7dda\u8010\u6027<\/strong>\u3092\u793a\u3059\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

💡 \u306a\u305c\u8010\u6027\u304c\u9ad8\u3044\u306e\u304b\uff1f<\/h3>\n\n\n\n

mAG \u306b\u306f\u3001\u767a\u5149\u4e2d\u5fc3\uff08\u30af\u30ed\u30e2\u30d5\u30a9\u30a2\uff09\u3068\u30d2\u30b9\u30c1\u30b8\u30f3\u6b8b\u57fa\u306e \u03c0\u30b9\u30bf\u30c3\u30ad\u30f3\u30b0\u69cb\u9020<\/strong>\u3068\u3044\u3046\u3001EGFP\u306b\u306f\u306a\u3044\u7279\u5fb4\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u69cb\u9020\u304c\u3001\u96fb\u5b50\u7dda\u306b\u3088\u308b\u5206\u89e3\u3092\u53d7\u3051\u306b\u304f\u3044\u3088\u308a\u201c\u58ca\u308c\u306b\u304f\u3044\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u201d\u3092\u5b9f\u73fe\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n

🌱 \u4e7e\u71e5\u72b6\u614b\u3068\u6eb6\u6db2\u4e2d\u306e\u9055\u3044<\/h3>\n\n\n\n

\u3055\u3089\u306b\u672c\u7814\u7a76\u3067\u306f\u3001\u4e7e\u71e5\u72b6\u614b<\/strong>\u3088\u308a\u3082\u6eb6\u6db2\u4e2d\uff08\u6c34\u4e2d\uff09\u306e\u307b\u3046\u304cmAG \u306e\u96fb\u5b50\u7dda\u8010\u6027\u304c\u3084\u3084\u9ad8\u3044\u3053\u3068\u3082\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001\u6eb6\u6db2\u4e2d\u3067\u306f\u5206\u5b50\u304c\u5468\u56f2\u3068\u4ea4\u63db\u3055\u308c\u3001\u69cb\u9020\u306e\u201c\u81ea\u5df1\u4fee\u5fa9\u201d\u304c\u90e8\u5206\u7684\u306b\u8d77\u304d\u3066\u3044\u308b\u53ef\u80fd\u6027<\/strong>\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

\n\n\n\n

🧬 Why mAzamiGreen?<\/h3>\n\n\n\n

In this study, Minoda Lab investigated mAzamiGreen (mAG)<\/strong>, a fluorescent protein known for its high structural stability<\/strong>, and compared it with the widely used EGFP. Electron\u2011beam irradiation caused: (1) a decrease in luminescence intensity, and (2) a red shift of the emission peak in both proteins. However, a key finding is that mAG survives electron irradiation more than one order of magnitude better than EGFP<\/strong>.<\/p>\n\n\n\n

💡 Origin of the enhanced resistance<\/h3>\n\n\n\n

mAG possesses a unique \u03c0\u2011stacking interaction<\/strong> between its chromophore and a histidine residue, a feature absent in EGFP. This structural robustness is considered responsible for the slower intensity decay and improved electron\u2011beam resistance<\/strong>.<\/p>\n\n\n\n

💧 Dry vs. wet environments<\/h3>\n\n\n\n

The experiments further revealed that mAG is slightly more resistant: in solution than in the dry state. This suggests that molecular exchange with the surrounding environment may allow partial structural repair under irradiation.<\/p>\n\n\n\n

🚀 Future potential<\/h3>\n\n\n\n

These results indicate that mAzamiGreen is a promising candidate for: (i) cathodoluminescence\u2011based correlative microscopy, (ii) multi\u2011color CL imaging, and (iii) real\u2011time observation of biological structures at nanometer resolution.<\/p>\n\n\n\n

JOURNAL PAPER: Micron
Volume 201, February 2026, 103944
Investigation of electron beam resistance of mAzamiGreen using an electron photon hybrid microscope
Mihiro Suzuki, Toshiki Shimizu, Hiroki Minoda
https:\/\/doi.org\/10.1016\/j.micron.2025.103944<\/a><\/p>\n\n\n\n

Fluorescent proteins allow scientists to \u201csee\u201d what is happening inside living cells using light. Electron microscopes, on the other hand, can observe extremely fine structures, but strong electron beams often damage fluorescent proteins. In this study, Minoda Lab focused on mAzamiGreen (mAG), a structurally stable fluorescent protein. They found that although electron beams reduce its brightness, mAG survives more than ten times longer than the commonly used EGFP. They also discovered that mAG is slightly more resistant in liquid than in dry conditions, likely because molecules can partially repair their structure. These results open the door to next\u2011generation microscopes that combine light and electron imaging in real time.<\/p>\n","protected":false},"excerpt":{"rendered":"

\u96fb\u5b50\u7dda\u3067\u86cd\u5149\u306f\u3069\u3053\u307e\u3067\u8010\u3048\u308b\uff1f
\nmAzamiGreen\u306e\u65b0\u3057\u3044\u53ef\u80fd\u6027\u3092\u89e3\u660e\u3002How resistant are fluorescent proteins to electron beams?
\nmAzamiGreen shows remarkable robustness.<\/p>\n","protected":false},"author":1,"featured_media":7773,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[9],"tags":[16],"class_list":["post-7772","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research","tag-measurement-devices"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/tuat-chemphys.net\/wp-content\/uploads\/2026\/04\/Designer-3.png?fit=1536%2C1024&ssl=1","jetpack_sharing_enabled":true,"jetpack-related-posts":[{"id":6478,"url":"https:\/\/tuat-chemphys.net\/?p=6478","url_meta":{"origin":7772,"position":0},"title":"Eco-friendly 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